Identification and characterization of differentially expressed genes from Fagus sylvatica roots after infection with Phytophthora citricola
Identifieur interne : 000E89 ( Main/Exploration ); précédent : 000E88; suivant : 000E90Identification and characterization of differentially expressed genes from Fagus sylvatica roots after infection with Phytophthora citricola
Auteurs : Katja Schlink [Allemagne]Source :
- Plant Cell Reports [ 0721-7714 ] ; 2009.
Abstract
Abstract: Phytophthora species are major plant pathogens infecting herbaceous and woody plants including European beech, the dominant or co-dominant tree in temperate Europe and an economically important species. For the analysis of the interaction of Phytophthora citricola with Fagus sylvatica suppression subtractive hybridization was used to isolate transcripts induced during infection and 1,149 sequences were generated. Hybridizations with driver and tester populations demonstrated differential expression in infected roots as compared to controls and verify efficient enrichment of these cDNAs during subtraction. Up regulation of selected genes during pathogenesis demonstrated using RT-PCR is consistent with these results. Pathogenesis-related proteins formed the largest group among functionally categorized transcripts. Cell wall proteins and protein kinases were also frequently found. Several transcription factors were isolated that are reactive to pathogens or wounding in other plants. The library contained a number of jasmonic acid, salicylic acid and ethylene responsive genes as well as genes directly involved in signaling pathways. Besides a mechanistic interconnection among signaling pathways another factor explaining the activation of different pathways could be the hemibiotrophic life style of Phytophthora triggering different signals in both stages.
Url:
DOI: 10.1007/s00299-009-0694-2
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001265
- to stream Istex, to step Curation: 001265
- to stream Istex, to step Checkpoint: 000304
- to stream Main, to step Merge: 000F07
- to stream Main, to step Curation: 000E89
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and characterization of differentially expressed genes from Fagus sylvatica roots after infection with Phytophthora citricola</title>
<author><name sortKey="Schlink, Katja" sort="Schlink, Katja" uniqKey="Schlink K" first="Katja" last="Schlink">Katja Schlink</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:15FE2D89E4D1B2CFBCC22752B9AE728F39191A76</idno>
<date when="2009" year="2009">2009</date>
<idno type="doi">10.1007/s00299-009-0694-2</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-KR6QXB1P-8/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001265</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001265</idno>
<idno type="wicri:Area/Istex/Curation">001265</idno>
<idno type="wicri:Area/Istex/Checkpoint">000304</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000304</idno>
<idno type="wicri:doubleKey">0721-7714:2009:Schlink K:identification:and:characterization</idno>
<idno type="wicri:Area/Main/Merge">000F07</idno>
<idno type="wicri:Area/Main/Curation">000E89</idno>
<idno type="wicri:Area/Main/Exploration">000E89</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Identification and characterization of differentially expressed genes from <hi rend="italic">Fagus sylvatica</hi>
roots after infection with <hi rend="italic">Phytophthora citricola</hi>
</title>
<author><name sortKey="Schlink, Katja" sort="Schlink, Katja" uniqKey="Schlink K" first="Katja" last="Schlink">Katja Schlink</name>
<affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Forest Genetics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Am Hochanger 13, 85354, Freising</wicri:regionArea>
<orgName type="university">Université technique de Munich</orgName>
<placeName><settlement type="city">Munich</settlement>
<region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Plant Cell Reports</title>
<title level="j" type="abbrev">Plant Cell Rep</title>
<idno type="ISSN">0721-7714</idno>
<idno type="eISSN">1432-203X</idno>
<imprint><publisher ref="https://scientific-publisher.data.istex.fr/ark:/67375/H02-SWLMH5L1-1">Springer-Verlag</publisher>
<pubPlace>Berlin/Heidelberg</pubPlace>
<date type="published" when="2009">2009</date>
<biblScope unit="vol" from="28" to="28">28</biblScope>
<biblScope unit="issue" from="5" to="5">5</biblScope>
<biblScope unit="page" from="873">873</biblScope>
<biblScope unit="page" to="882">882</biblScope>
</imprint>
<idno type="ISSN">0721-7714</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0721-7714</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Phytophthora species are major plant pathogens infecting herbaceous and woody plants including European beech, the dominant or co-dominant tree in temperate Europe and an economically important species. For the analysis of the interaction of Phytophthora citricola with Fagus sylvatica suppression subtractive hybridization was used to isolate transcripts induced during infection and 1,149 sequences were generated. Hybridizations with driver and tester populations demonstrated differential expression in infected roots as compared to controls and verify efficient enrichment of these cDNAs during subtraction. Up regulation of selected genes during pathogenesis demonstrated using RT-PCR is consistent with these results. Pathogenesis-related proteins formed the largest group among functionally categorized transcripts. Cell wall proteins and protein kinases were also frequently found. Several transcription factors were isolated that are reactive to pathogens or wounding in other plants. The library contained a number of jasmonic acid, salicylic acid and ethylene responsive genes as well as genes directly involved in signaling pathways. Besides a mechanistic interconnection among signaling pathways another factor explaining the activation of different pathways could be the hemibiotrophic life style of Phytophthora triggering different signals in both stages.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>Bavière</li>
<li>District de Haute-Bavière</li>
</region>
<settlement><li>Munich</li>
</settlement>
<orgName><li>Université technique de Munich</li>
</orgName>
</list>
<tree><country name="Allemagne"><region name="Bavière"><name sortKey="Schlink, Katja" sort="Schlink, Katja" uniqKey="Schlink K" first="Katja" last="Schlink">Katja Schlink</name>
</region>
<name sortKey="Schlink, Katja" sort="Schlink, Katja" uniqKey="Schlink K" first="Katja" last="Schlink">Katja Schlink</name>
<name sortKey="Schlink, Katja" sort="Schlink, Katja" uniqKey="Schlink K" first="Katja" last="Schlink">Katja Schlink</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E89 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E89 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= MelampsoraV2 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:15FE2D89E4D1B2CFBCC22752B9AE728F39191A76 |texte= Identification and characterization of differentially expressed genes from Fagus sylvatica roots after infection with Phytophthora citricola }}
This area was generated with Dilib version V0.6.38. |